lightswitch
BSc Thesis
Expression of twin-plant-inducing transgenes RKD1, RKD2 and RKD4 was optimized in Rosetta BL21 Escherichia coli cells. This was done by culturing bacteria of which proteins were isolated and subsequently analyzed using SDS-PAGE, western blotting and coomassie staining.

Here Escherichia coli is transformed with four plasmids of which three contain one of the RKD1, RKD2 or RKD4 plant transgenes. Next, E.coli cultures were selected and grown on a larger scale to generate glycerol stocks as well as plasmid isolation.

This shows the procedure to analyse at which temperature the expression of RKD1, RKD2, and RKD4 plant transgenes is most optimal in Escherichia coli. This was done to acquire the highest production of these genes for isolation and subsequent crystal structure analysis.